Fluorescence Spectrometer

Fluorescence Spectrophotometer

Fluorescence Spectrophotometer

      Fluorescence spectroscopy is a type of electromagnetic spectroscopy that analyzes fluorescence from a sample. It involves using a beam of light, usually ultraviolet light, that excites the electrons in molecules of certain compounds and causes them to emit light; typically, but not necessarily, visible light. A complementary technique is absorption spectroscopy.

      Fluorescence Spectrophotometer F-2700

     The Hitachi F-2700 Fluorescence Spectrophotometer is available in two configurations depending on your laboratory’s needs. The F-2700 can be used as stand-alone and also PC-controlled.

       The F-2700 in stand-alone configuration using the built-in keyboard and LCD screen is ideal for teaching and routine measurements. Adding PC control expands its capabilities to biotech research and quantum yield applications.

The large built-in keyboard and LCD screen make operation a breeze.
The large built-in keyboard and LCD screen make operation a breeze.

       The F-2700 feature an excellent signal-to-noise ratio (800 S/N RMS, Raman band of water at 5 nm bandwidth). With PC control the scan speed is as high as 12,000nm/min, making it ideal for 3-D scan measurements. A wide array of accessories is available, including automatic sampling, quantum yield measurement, full-range spectral correction, and 100µL micro cell.

       Hitachi's unique concave diffraction gratings combined with a low noise detection system result in an excellent signal-to-noise ratio (S/N ≥ 800 RMS, Raman band of water using 5nm slits), allowing measurement of fluorescein with sensitivity in the order of 1 x 10-12 mol/l. 

       Hitachi fluorescence spectrophotometers offer a wide dynamic range of 6 or more orders of magnitude. This is the result from our unique circuit-processing technology where our fluorescence spectrophotometers switch gain automatically and allow measuring samples with low and high fluorescence intensity under the same conditions.  This eliminates the need to prepare multiple calibration curves for a range of concentrations.

 

F-2700 Fluorescein detection sensitivity and wide dynamic range.
F-2700 Fluorescein detection sensitivity and wide dynamic range.

 

       The Hitachi F-2700 fluorescence spectrophotometers's variable slit width works well for samples displaying sharp peaks or for samples with broad band peaks.  

Left: Fluorescence spectrum of Y2O3 (slit 2.5nm)     Right: Spectrum of YAG fluorescence substance (slit 20nm)
Left: Fluorescence spectrum of Y2O3 (slit 2.5nm) Right: Spectrum of YAG fluorescence substance (slit 20nm)

 

       The F-2700 Fluorescence Spectrophotometer offers optional accessories for performing full range spectral correction for both the Excitation and Emission monochromators.  This allows eliminating spectral distortions in your data resulting from the variation in power distribution from lamp to lamp, efficiency curves of the diffraction gratings and sensitivity of the detector.  This is a desirable feature when publishing data or when comparing data from instrument to instrument.  The picture below shows a measurement for NIST SRM 936.

 

Measurement of NIST SRM936e before and after spectral correction
Measurement of NIST SRM936e before and after spectral correction

 

       Wide range of accessories to meet your application needs from Biology, Environmental, Pharmaceutical QC, Teaching and Materials Research.